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Study of volumetric oxygen transfer coefficient in a Taylor vortex flow bioreactor
Patrícia Ap. Santiago, Cláudio A. T. Suazo, Roberto C. Giordano. Federal University of São Carlos
An inadequate transfer of oxygen can lead to rapid changes in cell physiology, metabolism, and cell death. In this work, the KLa is evaluated in a Taylor vortex flow bioreactor and a mass transfer correlation is proposed. The experimental conditions employed were: rotational Reynolds number between 244 and 2928, airflow rates varying from 80 mL.min-1 to 550 mL.min-1 and used culture medium DMEM.
Hyperbaric Bioreactors use with Yarrowia Lipolytica Cultures: Cellular Adaptation to Hyperbaric Conditions
Marlene Lopes, Nelma Gomes, Manuel Mota, Isabel Belo. IBB-Institute for Biotechnology and Bioengineering, Center of Biological Engineering, University of Minho, Braga, Portugal
In this work, a pressurized bioreactor was used for Y. lipolytica batch cultivation under increased air pressure from 1 bar to 6 bar. Cell growth was strongly enhanced by the pressure rise. The increase of oxygen availability caused the induction of the antioxidant enzyme SOD. The pre-growth of Y. lipolytica under increased pressure conditions did not affect the lipase production ability of the cells.
Adaptive control of glycerol & methanol feeding in recombinant Pichia pastoris cultures: Impact on antibody titre
Filipe Ataíde, Ana Raquel Ferreira, João J. Clemente, António E. Cunha, Rui Oliveira. CQFB/REQUIMTE and IBET/ITQB
Pichia pastoris processes for heterolougous protein expression are typically run in three phases: a batch phase, an exponential feeding fed-batch phase and a long oxygen transfer limitation (OTL) phase. The final protein titre is often limited by the oxygen availability in the OTL phase. Here a direct adaptive controller is designed for the OTL phase. The controller was validated in pilot P. pastoris cultivations expressing a single chain antibody fragment (ScFv). This work shows that the propos
Palladium-Catalyzed Three-Component Synthesis of Phenothiazines
Troels Dahl, Christian Wenzel Tornøe, Benny Bang-Andersen, Poul Nielsen and Morten Jørgensen. H. Lundbeck A/S
The formation of aromatic carbon-heteroatom bonds has traditionally been achieved by nucleophilic aromatic substitution or via the copper-mediated Ullman reaction. The palladium-catalyzed formation of aromatic C-N bonds extensively developed by Hartwig and Buchwald has provided a powerful alternative. While the aryl amination reaction is applicable even to aryl chlorides and activated phenols, the analogous C-O and C-S bond forming reactions have attracted less attention.
Phase Tranformation of Sodium Carbonate to Monohydrate
A. Shaikh, A.D. Salman, S. Mcnamara, G, Littlewood and M.J. Hounslow. Sheffield University
Sodium carbonate is the most widely used chemical compound in the world. The 50% of this used in glass industry is in the form of dense sodium carbonate. The process of transformation of sodium carbonate to sodium carbonate monohydrate is the key step in the production of dense sodium carbonate. In this present work systematic experiments were conducted by using online in-situ measurement techniques. It is shown that the kinetics of phase transformation process is highly temperature dependant.
Application of Microarrays in Process Analytical Technologies A First Step Towards Real Time Product Release
Streefland M, van de Waterbeemd B, vander Pol LA, Beuvery EC, Martens DE,Tramper J. The Netherlands Vaccine Institute, Wageningen University, PAT Consultancy,
Application of microarrays during process development helps to gain insight in biological processes involved in product formation, increasing process understanding.
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